METHODS FOR SEPARATION, CHARACTERISTICS AND (OR) IDENTIFICATION OF MICROORGANISMS USING MASS SPECTROMETRY

摘要

1. A method for characterizing and / or identifying a microorganism from a test sample, comprising: (a) obtaining a test sample that is known to contain or may contain microorganisms; (b) selective lysis of non-microorganism cells in the test sample to obtain a lysed sample; (c) the separation of microorganisms from other components of the specified lysed sample with the formation of an isolated sample of the microorganism; (d) the study of the specified isolated sample of the specified microorganism using mass pektrometrii for removing the mass spectrum of this microorganism, and (e) characterization and / or identification of the microorganism in said isolated sample measured by comparing the mass spectrum with reference mass spectra and / or the known or predicted masses of cellular components known mikroorganizmov2. The method according to claim 1, wherein at least a portion of said isolated sample of microorganisms from step (c) is isolated and placed on a carrier substrate before the research step (d). The method according to claim 1, wherein the separation means in step (c) is centrifugation. The method according to claim 1, wherein the separation means in step (c) is filtration. The method according to claim 1, wherein at least a portion of an isolated sample of the microorganism from step (c) is deposited directly on the carrier substrate during separation prior to the study step (d). The method according to claim 1, wherein said mass spectrometry is MALDI-TOF mass spectrometry, electrospray desorption ionization (DESI) mass spectrometry, gas chromatography mass spectrometry, liquid chromatography mass spectrometry, �