BINDING PROTEINS INHIBITING VEGF-A RECEPTOR INTERACTIONS

摘要

1. A recombinant binding protein containing at least one repeat domain, wherein the repeat domain binds VEGF-Axxx to Book 10M and inhibits the binding of VEGF-Axxx to VEGFR-2.2. The binding protein according to claim 1, wherein said repeated domain inhibits the germination of HUVEC spheroids with an I less than 10 nM. 3. The binding protein according to claim 1, wherein K for the interaction of the specified repeated domain with VEGF-Axxxb is at least 10 times higher compared to K for the interaction of the specified repeated domain with the corresponding VEGF-Axxx. The binding protein of claim 1, wherein said repeat domain is an ankyrin repeat domain. The binding protein according to claim 4, wherein said ankyrin repeat domain comprises a repeat module with an ankyrin repeat motif of the sequence ID23GWTPLHL4ADLG5LEIVEVLLK6GADVN7 (SEQ ID NO: 4), where1 is an amino acid residue selected from the group consisting of K, T and Y; 2 is an amino acid residue N or M; 3 represents an amino acid residue T or F; 4 represents an amino acid residue S or A; 5 represents an amino acid residue H or R; 6 represents an amino acid residue selected from the group consisting of of A, Y, H, and N, i7 represents an amino acid residue is A or T.6. The binding protein of claim 4, wherein said ankyrin repeat domain includes a repeat module with an ankyrin repeat motif of the sequence 1D23G4TPLHLAA56GH7EIVEVLLK8GADVNA (SEQ ID NO: 5), where 1 is an amino acid residue selected from the group consisting of A, N, R, V, Y, E, H, I, K, L, Q, S, and T; 2 represents an amino acid residue selected from the group consisting of S, A, N, R, D, F, L, P, T, and Y; 3 represents self amino