STABLE VECTOR OF CONSTITUTIONALLY HIGH EXPRESSION FOR OBTAINING HPV VACCINES AND RECOMBINANT LACTIC-ACID BACTERIA TRANSFORMED WITH THIS VECTOR

摘要

1. The surface expression vector for the production of vaccines against human papillomavirus (HPV), including a gene encoding a mutant protein repE having the amino acid sequence of SEQ ID NO: 1, a promoter, a complex gene of poly-gamma-glutamate synthetase and a gene that is linked to a complex gene poly-gamma-glutamate synthetase and encodes an antigenic protein of the human papillomavirus associated with tumor induction. 2. The surface expression vector of claim 1, wherein the HPV antigen protein associated with the induction of the tumor is HPV E7 or E7 (Rb) protein. The surface expression vector according to claim 1, wherein the promoter is an aldolase promoter derived from lactic acid bacteria. The surface expression vector of claim 1, wherein the poly-gamma-glutamate synthetase complex gene is selected from the group consisting of pgsB, pgsC and pgsA. The recombinant microorganism transformed by the vector according to claim 1.6. The recombinant microorganism according to claim 1, which is a lactic acid bacteria. A vaccine for the treatment of cervical cancer, comprising a transformed recombinant microorganism according to claim 5 or 6 as an active ingredient. A method for producing a microorganism containing an HPV antigen that is expressed on its surface, the method comprising the steps of: cultivating a recombinant microorganism according to claim 5 for expressing an HPV antigen on the surface of a microorganism; and collecting a recombinant microorganism containing the HPV antigen that is expressed on its surface. A vaccine for the treatment of cervical cancer, including a transformed recombinant microorganism containing the HPV antigen as an active ingredient,