WAYS TO REDUCE VIRULICIDAL ACTIVITY CONTAINING PCV-2 COMPOSITIONS AND CONTAINING PCV-2 COMPOSITIONS WITH IMPROVED IMMUNOGENICITY

摘要

1. The method of obtaining PCV-2-containing antigenic composition, which consists in the fact that carry out the stages in which: I) receive the first liquid containing PCV-2 antigen; and ii) at least a portion of the first liquid is separated from the PCV-2.2 antigen. The method of claim 1, wherein a portion of the first fluid is separated from the PCV-2 antigen by replacing a portion of the first fluid with a second fluid, wherein the second fluid is different from the first fluid. The method according to claim 2, wherein replacing a portion of the first liquid with a second liquid comprises the steps of: a) adding liquid by adding a second liquid to the first liquid containing PCV-2 antigen; ib) concentrate PCV-2 antigen by separating a portion of the first and second fluids. The method according to one of claims 1 to 3, in which a portion of the first liquid is separated from the PCV-2 antigen by performing a filtration step using a filter. The method according to claim 3, in which the stage of concentration and the stage of adding liquid is carried out almost simultaneously. The method according to claim 3, wherein the concentration step and the liquid addition step are carried out at least two times. The method of claim 4, wherein the filter comprises a semipermeable membrane. The method of claim 7, wherein the semipermeable membrane has an average pore size that is smaller than the size of the PCV-2 antigen, which prevents at least 90% of the PCV-2 antigen from passing through the pores of the semipermeable membrane and retains the PCV-2 antigen on the filter. The method according to claim 4, wherein the filter has such an average pore size that prevents the passage of at least 90% of proteins with a molecular weight of from 50 to 500 kDa. The method according to claim 3, in which at the stage of concentration