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Saccharomyces cerevisiae YEAST STRAIN, HAVING AMYLASE ACTIVITY, FOR PRODUCING FEED PROTEIN PRODUCT AND METHOD OF PRODUCING FEED PROTEIN PRODUCT

机译:具有淀粉酶活性的啤酒酵母酵母,用于生产饲料蛋白产品和生产饲料蛋白产品的方法

摘要

FIELD: chemistry.;SUBSTANCE: group of inventions relates to feed production, particularly a method for microbiological production of feed yeast from grain wastes. The method involves preparation of grain material by grinding to 120-160 mcm particles. Further, the ground grain material is used to prepare an aqueous suspension containing 15-25% dry substances. The obtained suspension is saccharified with α-amylase and glucoamylase to 6-10% glucose content in the suspension. Nitrogen and phosphorus sources are added to the obtained suspension such that 90 mg of nitrogen and 45 mg of phosphorus are required for synthesis of 1 g of biomass. A culture is added to the obtained nutrient medium, said culture being a producer of the Saccharomyces cerevisiae yeast strain VKPM U-3585, having amylase activity, which is deposited in the Russian National Collection of Industrial Microorganisms (VKPM) and can be used in producing feed protein. The Saccharomyces cerevisiae yeast strain VKPM U-3585 is continuously grown in a multiple-section apparatus while feeding the nutrient medium simultaneously into several sections, followed by concentration of the suspension by vacuum evaporation and drying. Moisture freed at the vacuum evaporation and drying steps is used to prepare the aqueous suspension of grain material.;EFFECT: invention increases output of crude protein and true protein.;5 ex
机译:发明领域本发明涉及饲料生产,特别是从谷物废物中微生物生产饲料酵母的方法。该方法涉及通过研磨至120-160mcm的颗粒来制备谷物材料。此外,将磨碎的谷物材料用于制备包含15-25%干物质的水性悬浮液。将获得的悬浮液用α-淀粉酶和葡糖淀粉酶糖化至悬浮液中的葡萄糖含量为6-10%。将氮和磷源添加到获得的悬浮液中,以便合成1 g生物质需要90 mg氮和45 mg磷。将培养物添加到获得的营养培养基中,所述培养物是酿酒酵母酵母菌株VKPM U-3585的生产者,该菌株具有淀粉酶活性,并保存在俄罗斯国家工业微生物收藏中(VKPM),可用于生产饲料蛋白质。酿酒酵母酵母菌株VKPM U-3585在多部分装置中连续生长,同时将营养培养基同时分成几个部分,然后通过真空蒸发和干燥浓缩悬浮液。在真空蒸发和干燥步骤中释放的水分用于制备谷物材料的水悬浮液。效果:本发明增加了粗蛋白和真蛋白的产量。5

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