STABLE AND SELECTIVE FORMATION OF HOOGSTEEN-TYPE TRIPLEXES AND DUPLEXES USING TWISTED INTERCALATING NUCLEIC ACIDS (TINA) AND METHOD FOR PREPARATION OF TINA

摘要

FIELD: chemistry.;SUBSTANCE: flexible monomer contains a skeleton monomer unit of an oligonucleotide or an oligonucleotide analogue which can be linked to two cyclic ring systems by an alkylenediol and a linker. The ring systems are linked to each other by a conjugator. The flexible monomer can be adapted for incorporation into an oligonucleotide. The method of producing the oligonucleotide involves embedding the flexible monomer during oligonucleotide synthesis, said flexible monomer increasing base-stacking and being adapted for incorporation into the oligonucleotide. The oligonucleotide may contain one or more flexible monomers which increase base-stacking.;EFFECT: increasing base-stacking.;12 cl, 6 dwg, 4 tbl, 10 ex