METHOD FOR DETERMINING AETIOLOGY OF TONSILLITIS IN CHILDREN

摘要

FIELD: medicine.;SUBSTANCE: biomaterial is sampled at the height of the disease. That is ensured by tonsil scraping. The biomaterial is placed in a 1.5 ml disposable capped plastic tube, and 0.9% sodium chloride 1.0 ml is added; then the biomaterial is centrifuged; the supernatant is collected, and the residue is re-suspended in a buffer solution cooled to +4°C, to the final cell concentration of 5×10⁶-10×10⁶ cell/ml. Then the cell suspension 100 mcl is placed in a cytometric tube on the ice bath; annexin V-FITC 10 mcl and 7AAD 20 mcl are added; the 15-minute incubation is followed by adding the buffer solution 400 mcl cooled to +4°C, and mixed in a vibration mixer. The annexin V-stained and 7AAD-unstained cell are relatively counted in a flow cytometre. Provided that the tonsil scraping contains 16-40% of apoptosis mononuclears, a bacterial genesis of tonsillitis is stated, while the mononuclear count being 41% or more shows the viral tonsil involvement caused by infectious mononucleosis.;EFFECT: method improvement.;2 ex, 1 tbl