Cell differentiation in samples using extracellular enzyme inactivation before intracellular enzyme release

摘要

Being the method of detecting the presence of the target cell in the liquid sample:(A) the aforementioned sample: Including the nutrient medium outside the cell which includes the enzyme which possesses (i) measurable activity it becomes; At the same time there is the doubt which includes the cell endoenzyme target cell which possesses (ii) aforementioned measurable activity;(B) the aforementioned method: It inactivates the aforementioned measurable activity inside the nutrient medium outside the (i) aforementioned cell, but as for the aforementioned measurable activity in the aforementioned target cell with the reagent which is not inactivated, the process which processes the aforementioned liquid sample; Melting the (ii) aforementioned target cell, the process which makes the aforementioned cell endoenzyme discharge; And including the process which measures (iii) aforementioned measurable activity, it becomes. Because of this, it is possible to measure the cell endoenzyme without receiving the interference by the exoenzyme. This invention is suitable especially although the bacterial infection blood is processed making use of the detection assay which is based on adenylic acid kinase activity. Selective figure Figure 1