ROOT SOAKING METHOD TO PRODUCE DOUBLE HAPLOID INBRED LINES IN CORN

摘要

The subject invention pertains to a method of generating double haploid (DH) corn inbred lines from haploid corn plants. The roots of haploid corn plants are contacted with a solution/composition comprising about 0.0015M colchicine and about 0.11M dimethyl sulfoxide and the treated haploid corn plants are grown. DH corn plants are selected from the treated haploid corn plants and the selected DH corn plants are used to produce DH corn inbred lines. The present invention also provides a method for increasing the DH success rate with corn plants.