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Multiplex Compositions And Methods For Quantification Of Human Nuclear DNA And Human Male DNA And Detection of PCR Inhibitors

机译:多重组合物,人类核DNA和人类男性DNA的定量方法以及PCR抑制剂的检测

摘要

The invention relates to a method for simultaneous quantification of human nuclear DNA and human male DNA in a biological sample while also detecting the presence of PCR inhibitors in a single reaction. The multiplex quantification method also provides a ratio of human nuclear and male DNA present in a biological sample. Such sample characterization is useful for achieving efficient and accurate results in downstream molecular techniques such as genotyping.
机译:本发明涉及一种在生物样品中同时定量人类核DNA和人类男性DNA,同时在单个反应中检测PCR抑制剂的存在的方法。多重定量方法还提供了生物样品中存在的人类核DNA和男性DNA的比例。这种样品表征对于在下游分子技术(例如基因分型)中获得有效和准确的结果很有用。

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