TRANSFORMANT OF YEAST OF GENUS SCHIZOSACCHAROMYCES, METHOD FOR PRODUCING SAME, METHOD FOR PRODUCING ß-GLUCOSIDASE, AND CELLULOSE DEGRADATION METHOD

摘要

To provide a transformant of a yeast of the genus Schizosaccharomyces which can produce a modified-type β-glucosidase with improved glucosidase activity, a method for producing such a transformant, a method for producing the modified-type β-glucosidase, and a cellulose degradation method using the modified-type β-glucosidase. A transformant of a yeast of the genus Schizosaccharomyces characterized by having a structural gene sequence comprising a region encoding a modified-type β-glucosidase which is obtained by introducing at least one mutation selected from the group consisting of specific mutations into a β-glucosidase derived from a filamentous fungus, and a promoter sequence and a terminator sequence for expressing the structural gene in a chromosome, or alternatively by having the sequences as an extrachromosomal gene, and is characterized in that the modified-type β-glucosidase has higher thermostability or higher resistance to glucose inhibition than a β-glucosidase which does not have the specific mutations.