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TRANSFORMANT OF YEAST OF GENUS SCHIZOSACCHAROMYCES, METHOD FOR PRODUCING SAME, METHOD FOR PRODUCING BETA-GLUCOSIDASE, AND METHOD FOR DECOMPOSING CELLULOSE
TRANSFORMANT OF YEAST OF GENUS SCHIZOSACCHAROMYCES, METHOD FOR PRODUCING SAME, METHOD FOR PRODUCING BETA-GLUCOSIDASE, AND METHOD FOR DECOMPOSING CELLULOSE
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机译:裂殖酵母属酵母的转化体,其制备方法,β-葡糖苷酶的制备方法以及纤维素的分解方法
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摘要
To provide a transformant of a yeast of the genus Schizosaccharomyces which can produce a modified-type ²-glucosidase with improved glucosidase activity, a method for producing such a transformant, a method for producing the modified-type ²-glucosidase, and a cellulose degradation method using the modified-type ²-glucosidase. A transformant of a yeast of the genus Schizosaccharomyces characterized by having a structural gene sequence comprising a region encoding a modified-type ²-glucosidase which is prepared by introducing at least one mutation selected from the group consisting of specific mutations into a ²-glucosidase derived from a filamentous fungus, and a promoter sequence and a terminator sequence for expressing the structural gene in a chromosome, or alternatively by having the sequences as an extrachromosomal gene, and is characterized in that the modified-type ²-glucosidase has higher thermostability or higher resistance to glucose inhibition than a ²-glucosidase which does not have the specific mutations.
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