首页> 外国专利> TRANSFORMANT OF YEAST OF GENUS SCHIZOSACCHAROMYCES, METHOD FOR PRODUCING SAME, METHOD FOR PRODUCING BETA-GLUCOSIDASE, AND METHOD FOR DECOMPOSING CELLULOSE

TRANSFORMANT OF YEAST OF GENUS SCHIZOSACCHAROMYCES, METHOD FOR PRODUCING SAME, METHOD FOR PRODUCING BETA-GLUCOSIDASE, AND METHOD FOR DECOMPOSING CELLULOSE

机译:裂殖酵母属酵母的转化体,其制备方法,β-葡糖苷酶的制备方法以及纤维素的分解方法

摘要

To provide a transformant of a yeast of the genus Schizosaccharomyces which can produce a modified-type ²-glucosidase with improved glucosidase activity, a method for producing such a transformant, a method for producing the modified-type ²-glucosidase, and a cellulose degradation method using the modified-type ²-glucosidase. A transformant of a yeast of the genus Schizosaccharomyces characterized by having a structural gene sequence comprising a region encoding a modified-type ²-glucosidase which is prepared by introducing at least one mutation selected from the group consisting of specific mutations into a ²-glucosidase derived from a filamentous fungus, and a promoter sequence and a terminator sequence for expressing the structural gene in a chromosome, or alternatively by having the sequences as an extrachromosomal gene, and is characterized in that the modified-type ²-glucosidase has higher thermostability or higher resistance to glucose inhibition than a ²-glucosidase which does not have the specific mutations.
机译:为了提供裂殖酵母属的酵母的转化体,其可以产生具有改进的葡糖苷酶活性的修饰型β-葡糖苷酶,一种生产这种转化体的方法,一种制备修饰型β-葡糖苷酶的方法,以及纤维素降解。方法使用修饰的β-葡萄糖苷酶。裂殖酵母属酵母的转化体,其特征在于具有包含编码修饰型β-葡糖苷酶的区域的结构基因序列,该结构基因序列是通过将至少一种选自特定突变的突变引入由β-葡糖苷酶衍生的组中而制备的丝状真菌,以及用于在染色体上表达结构基因的启动子序列和终止子序列,或者通过具有作为染色体外基因的序列,其特征在于修饰型β-葡糖苷酶具有更高的热稳定性或更高的比没有特定突变的2-葡萄糖苷酶具有更高的抗葡萄糖抑制能力。

著录项

  • 公开/公告号EP2703484A4

    专利类型

  • 公开/公告日2014-12-31

    原文格式PDF

  • 申请/专利权人 ASAHI GLASS COMPANY LIMITED;

    申请/专利号EP20120760334

  • 发明设计人 OKADA KATSUNORI;TOHDA HIDEKI;

    申请日2012-03-19

  • 分类号C12N15/09;C12N1/19;C12N9/42;C12P19/14;

  • 国家 EP

  • 入库时间 2022-08-21 15:06:12

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