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TRANSFORMANT OF YEAST OF GENUS SCHIZOSACCHAROMYCES, METHOD FOR PRODUCING SAME, METHOD FOR PRODUCING β-GLUCOSIDASE, AND METHOD FOR DECOMPOSING CELLULOSE
TRANSFORMANT OF YEAST OF GENUS SCHIZOSACCHAROMYCES, METHOD FOR PRODUCING SAME, METHOD FOR PRODUCING β-GLUCOSIDASE, AND METHOD FOR DECOMPOSING CELLULOSE
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机译:裂殖酵母属酵母的转化体,其制备方法,β-葡糖苷酶的制备方法和纤维素的分解方法
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摘要
The purpose of the invention is to provide a transformant of yeast of the genus Schizosaccharomyces capable of producing a modified-type β-glucosidase with improved glucosidase activity, a method for producing the transformant, a method for producing the modified-type β-glucosidase, and a method for decomposing cellulose using the modified-type β-glucosidase. The transformant of yeast of the genus Schizosaccharomyces has a structural gene sequence containing a coding region for a modified-type β-glucosidase obtained by introducing at least one mutation among specific mutations into a filamentous fungus-derived β-glucosidase, and a promoter sequence and a terminator sequence for expressing the structural gene in a chromosome or as an extrachromosomal gene, and is characterized in that the modified-type β-glucosidase has higher thermostability or higher resistance to glucose inhibition than a β-glucosidase which does not have the specific mutations.
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