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GENOME SHUFFLING METHOD FOR AUTOGAMOUS PLANTS UTILIZING DOMINANT MALE STERILITY OBTAINED BY GENE ENGINEERING TECHNIQUE, AND RECURRENT SELECTION BREEDING SYSTEM BASED ON THE GENOME SHUFFLING METHOD
GENOME SHUFFLING METHOD FOR AUTOGAMOUS PLANTS UTILIZING DOMINANT MALE STERILITY OBTAINED BY GENE ENGINEERING TECHNIQUE, AND RECURRENT SELECTION BREEDING SYSTEM BASED ON THE GENOME SHUFFLING METHOD
A genome shuffling method for autogamous plants, including producing individuals having the following three traits in a tight coupling linkage by a gene engineering technique selected from a transgenic technique and a gene targeting technique: 1) dominant male sterility, 2) chemical tolerance and 3) lethality inducible by activating an inducible promoter, selecting, from progeny of the individuals, male-sterile individuals by means of the chemical tolerance described in 2) and male-fertile individuals by means of the lethality described in 3), arranging the male-sterile individuals and the male-fertile individuals close together in flowering periods thereof, so that the male-sterile individuals are crossed with the male-fertile individuals, harvesting seeds from the male-sterile individuals, and repeating outcrossing using the seeds from generation to generation.
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