Human hybrid host cell for mammalian gene expression

摘要

the tal lm w a n t s rmazu00f3 embriu00f3vesu00e9bu0151l hum and burkitt's cell - cell with no fu00e9lelimfu00f3m from rmazu00f3 fu00faziu00f3j out llu00edtott cells, tov bb procedure selu0151 llu00edt r and ra.hum hum a n / n hibridsejteket llu00edtottak out no hum nembriu00f3bu00f3l rmazu00f3 vesesejtek (2935) and modified, burkitt - fu00e9lelimfu00f3masejtek (2b8) with fu00faziu00f3j.the fuzion ltatott alkalmasgazdasejtek emlu0151sgu00e9nek rekombin ns cells express ra l s.this hkb - kneknevezett,hum a n and b cells with vesesejtek fu00faziu00f3j generated may be used hibridklu00f3nok emlu0151sgu00e9nek express l in s k with j r "vetkezu0151elu0151ny": (i) the cells didn't express immunoglobulin lnak,(ii) asejteket r zatott sejttenyu00e9sztu0151 in pots or fermentorban, suspension for tenyu00e9szetku00e9nt, plazmaeredetu0171 proteinektu0151l free insulin (ns tpoldatban rekombin dispenses with sa.with or without ", it can be propagated nynyen) k, (iii) the cells are susceptible to dna vu00e9gzetttranszfekciu00f3ra yes,the cells, and (iv) a heterologous rekombin nsproteineket - like pu00e9ld ul rekombin ns monoklon lis antibodies, soluble icam - 1, and rfviii - at - large quantities of sekret lj, k.