Provided are full-length cDNA clones of Langat tick-borne flavivirus. In particular, the strains TP21 and E5 were obtained as full-length clones in single plasmids. Production of virions from cloned full length cDNA resulted in variants wih reduced neurovirulence. Deletions in the 3' NCR also decreased neurovirulence. The attenuated variants of Langat tick-borne flavivirus may be used for vaccination purposes.
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