preparation immunoczujnika and its application to detect influenza virus

摘要

the subject of the invention is the preparation immunoczujnika to detect influenza virus, where pure gold electrodes shall be rinsed with ethanol and placed in a 10 mm ethanolic solution of 1,6 - heksanoditiolu (hdt) for 20 h at room temperature.then the washed sequentially with ethanol and deionised water and turns down the mountains and nakrapla 10? l gold colloidal solution (gcp guideline stated) to 18 h + 4u00b0c, in the next stage the electrodes shall be rinsed with pbs (0.1 m 145 mm nacl, 2.7 mm kcl, 8.4 mm nah2po4, na2hpo4 and 1.6 mm) nakrapla 10? l solution anti - m concentration of 28? g / ml in 0.1 m pbs buffer for 20 h + 4u00b0c.in the last stage of the electrode surfaces nakrapla 10? l solution 0.5% (w / v) of bsa in 0.1 m pbs for 2 h + 4u00b0c, as prepared electrodes shall be rinsed with pbs and stored at + 4u00b0c ready to use (no longer than one day). in addition, the application immunoczujnika revealed detection in solution protein m and influenza virus.