Procedure for replication of influenza viruses in cell culture and influenza viruses obtainable by the process

摘要

A process for making a vaccine for administration to humans or animals comprising: a) replication of influenza viruses in cell culture, in which cells that can be infected by influenza viruses are cultured in cell culture in suspension in serum free medium, the cells are infected with influenza viruses and after infection are cultured at a temperature in the range of 32º C to 34º C for virus replication, wherein a protease is added to the cultured cells before, during or after infection with influenza virus; b) collecting and isolating the influenza viruses replicated 2 to 10 days after infection, in which the cells or cell residues are separated from the culture medium; c) the formulation of influenza viruses to give the vaccine replicates, wherein: the infectivity of the replicated virus is destroyed by chemical and / or physical or viruses present in the vaccine as live attenuated viruses.