Detection and quantification of microbial multiplex nucleic acids internally controlled

摘要

A method for detecting and quantifying a microbial nucleic acid in a biological sample, said method comprising: a) providing a reaction mixture comprising one or more nucleic acids quantitative standards, two or more primer pairs, said pairs of primers specific for different sequence portions of said microbial nucleic acid, and one or more pairs of primers specific for said one or more standard nucleic acid, b) adding said biological sample to said reaction mixture, c) performing one or more cycles in a cycle comprising an amplification step, and said amplification step comprises obtaining two or more products of different amplification derived from said microbial nucleic acid if present in said sample to produce one or more amplification products derived from said one or more nucleic acids quantitative standards d) detecting and measuring the detectable signals generated by said amplification products and are 15 proportional to its concentration in said reaction mixture, wherein the presence or absence of the detectable signal or signals generated by said microbial nucleic acid is indicative of the presence or absence of microbial nucleic acid said biological e) shows determine the amount of said microbial nucleic acid in said biological sample compared to the different signals generated by said microbial nucleic acid and said one or more nucleic acids quantitative standards, in which said two or more portions of stream microbial nucleic acid are LTR and HIV GAG.