Process for enzymatic hydrolysis of lignocellulosic feedstocks pretreated

摘要

A process for enzymatic hydrolysis of a pretreated lignocellulosic feedstock to soluble sugars, the process comprising adding a mixture of cellulase enzymes to lignocelulósicapretratada raw material, said cellulase enzyme mixture comprising a mixture of primary cellulases decelobiohidrolasas CBH1 and CBH2 and endoglucanases EG1 and EG2, such cellobiohydrolases CBH1 yCBH2 being present at greater than or equal to 55% and less than 85% of the mixture of primary cellulases by weight, being presentedicha CBH2 at a fraction by weight relative to the cellobiohydrolase CBH1 and CBH2 such as defines C2 and the estandopresente said EG2 to a fraction by weight relative to EG1 and EG2 endoglucanases as defined E2, endonde when said CBH1 and CBH2 cellobiohydrolases are present above or equal to 55% and less than 65% delas primary cellulases by weight, said C2 values ​​are greater than or equal to 0.35 and less than 0.45 and said E2 values ​​are Superi pray to oiguales to 0.15 and less than 0.35; said f C2 values ​​are greater than or equal to 0.45 and less than 0.55 and said E2 values ​​greater than or equal a0.15 and less than 0.25; or those values ​​fc2 are greater than or equal to 0.45 and less than 0.55 and said E2 values ​​are greater than oiguales to 0.35 and less than 0.45, when said CBH1 and CBH2 cellobiohydrolases are present above or equal to 65% and under 75% primary cellulases by weight, said C2 values ​​are greater than or equal to 0.25 and less than 0.35 and said f E2 values ​​are greater than oiguales to 0.05 and less than 0.35; said f C2 values ​​are greater than or equal to 0.35 and less than 0.45 and said E2 values ​​are greater than oiguales to 0.05 and less than 0.55; said f C2 values ​​are greater than or equal to 0.45 and less than 0.65 and said E2 values ​​are greater than oiguales to 0.05 and less than 0.65; or said C2 values ​​are greater than or equal to 0.65 and less than 0.75 and said f E2 values ​​are greater than oiguales to 0.15 and less than 0.55, when said CBH1 and CBH2 cellobiohydrolases are present above or equal to 75% and less than 85% primary cellulases by weight, said C2 values ​​are greater than or equal to 0.35 and less than 0.45 and said E2 values ​​are greater than oiguales to 0.05 and less than 0.25; said f C2 values ​​are greater than or equal to 0.45 and less than 0.55 and said E2 values ​​are greater than oiguales to 0.15 and less than 0.65; or said C2 values ​​are greater than or equal to 0.55 and less than 0.65 and said f E2 values ​​are greater than oiguales to 0.15 and less than 0.35 and wherein the primary cellulases are from a fungal source.