SITE SPECIFIC INCORPORATION OF KETO AMINO ACIDS INTO PROTEINS

摘要

Compositions and methods of producing components of protein biosyntheticmachinery that include orthogonal tRNAs, orthogonal aminoacyl-tRNAsynthetases, and orthogonal pairs of tRNAs/synthetases, which incorporate ketoamino acids into proteins are provided. Methods for identifying theseorthogonal pairs are also provided along with methods of producing proteinswith keto amino acids using these orthogonal pairs.