Position Specific Incorporation of Nonnatural Amino Acids into Proteins In Vivo

摘要

The protein biosynthesis methods including nonnatural amino acids add new properties to proteins. And, the nonnatural proteins are valuable for analyzing their structure and function. The incorporation of nonnatural amino acids has accomplished by using an suppressor tRNA carrying the nonnatural amino acid to a ribosome associated with mRNA containing an expanded codon/anticodon pair (reviewed in) However, the incorporation of nonnatural amino acids in cells is difficult.