PROTEIN IN WHICH ELECTROSTATIC INTERACTION IS INTRODUCED WITHIN HYDROPHOBIC INTERACTION SITE AND PREPARATION METHOD THEREOF

摘要

The present invention provides a protein or antibody in which, of a pair of hydrophobic amino acids selected from within a hydrophobic interaction site of the protein, one hydrophobic amino acid is transformed into a substance having a positive electrical charge and the other hydrophobic amino acid is transformed into a substance having a negative electrical charge, and electrical interaction is introduced within the hydrophobic interaction site of the protein by means of the positive electrical charge and the negative electrical charge. The present invention also provides a method for preparing the protein or antibody, and a method for measuring the degree of coupling between a heavy chain and a light chain, using the antibody. The protein or antibody according to the present invention has a low contamination by a homodimer or a monomer, and thus a heterodimer can be obtained in high purity.