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Protein in which electrostatic interaction is introduced within hydrophobic interaction site and preparation method thereof
Protein in which electrostatic interaction is introduced within hydrophobic interaction site and preparation method thereof
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机译:在疏水相互作用位点内引入了静电相互作用的蛋白质及其制备方法
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摘要
The present invention relates to a hydrophobic interaction between a hydrophobic amino acid and a hydrophobic amino acid selected from the group consisting of a hydrophobic amino acid having a positive charge and a hydrophobic amino acid having a negative charge, And a protein or antibody into which an electrical interaction is introduced into the hydrophobic interaction site of the protein by the charge and the negative charge. The present invention also provides a method for producing the above protein or antibody, and a method for measuring the degree of coupling between a heavy chain and a light chain using the antibody. The protein or antibody according to the present invention is less contaminated with the homologous duplex or the monomer, and thus a heterodimer can be obtained with high purity.
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