sn-glycero-3-phosphocholine to CoA + 1,2-diacyl-sn-glycero-3-phosphocholine (EC 2.3.1.23) is disclosed herein to be over-expressed along with the over-expression of phospholipid:diacylglycerol acyltransferase ["PDAT"] having the ability to transfer a fatty acyl group from the sn-2 position of a phospholipid (e.g., phosphatidylcholine) to the sn-3 position of 1,2-diacylglycerol [E.C.2.3.1.158], thus resulting in a lysophospholipid and TAG. Co-expression of these enzymes in a recombinant microbial host cell resulted in increased production of long chain polyunsaturated fatty acids ["PUFAs"]."/>
IMPROVED PRODUCTION OF POLYUNSATURATED FATTY ACIDS BY COEXPRESSION OF ACYL-CoA:LYSOPHOSPHATIDYLCHOLINE ACYLTRANSFERASES AND PHOSPHOLIPID:DIACYLGLYCEROL ACYLTRANSFERASES
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IMPROVED PRODUCTION OF POLYUNSATURATED FATTY ACIDS BY COEXPRESSION OF ACYL-CoA:LYSOPHOSPHATIDYLCHOLINE ACYLTRANSFERASES AND PHOSPHOLIPID:DIACYLGLYCEROL ACYLTRANSFERASES
IMPROVED PRODUCTION OF POLYUNSATURATED FATTY ACIDS BY COEXPRESSION OF ACYL-CoA:LYSOPHOSPHATIDYLCHOLINE ACYLTRANSFERASES AND PHOSPHOLIPID:DIACYLGLYCEROL ACYLTRANSFERASES
Acyl-CoA:lysophosphatidylcholine acyltransferase ["LPCAT"] having the ability to convert acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine to CoA + 1,2-diacyl-sn-glycero-3-phosphocholine (EC 2.3.1.23) is disclosed herein to be over-expressed along with the over-expression of phospholipid:diacylglycerol acyltransferase ["PDAT"] having the ability to transfer a fatty acyl group from the sn-2 position of a phospholipid (e.g., phosphatidylcholine) to the sn-3 position of 1,2-diacylglycerol [E.C.2.3.1.158], thus resulting in a lysophospholipid and TAG. Co-expression of these enzymes in a recombinant microbial host cell resulted in increased production of long chain polyunsaturated fatty acids ["PUFAs"].
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机译：酰基-CoA：溶血磷脂酰胆碱酰基转移酶[“ LPCAT”]具有将酰基-CoA + 1-酰基- sn -甘油-3-磷酸胆碱转化为CoA + 1,2-二酰基-的能力本文公开了sn-甘油-3-磷酸胆碱（EC 2.3.1.23）与具有转移脂肪酰基的能力的磷脂：二酰基甘油酰基转移酶[“ PDAT”]一起过表达。从磷脂（例如磷脂酰胆碱）的 sn -2位置到1,2-二酰甘油的 sn -3位置[EC2.3.1.158]溶血磷脂和TAG。这些酶在重组微生物宿主细胞中的共表达导致长链多不饱和脂肪酸[PUFAs]的产量增加。
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