Overexpression of an endogenous type 2 diacylglycerol acyltransferase in the marine diatom Phaeodactylum tricornutum enhances lipid production and omega-3 long-chain polyunsaturated fatty acid content

摘要

Overexpression of DGAT2 isoforms in . Fatty acid composition (Mol%) of independent transgenic lines overexpressing DGAT2 genes during S phase. Each measurement is the average of three technical replicas (± standard error). Neutral lipid content of transgenic cells overexpressing different DGAT2 isoforms assessed by Nile Red fluorescence. Each data point represents an average of three biological replicas. Quantitative RT-PCR analysis of the transcript levels of DGAT2 isoforms. Transgenic algal cultures overexpressing , and were grown under continuous light for 3 days in standard F/2 medium. Relative expression levels were normalised to that of the housekeeping gene