首页> 外国专利> HSP70 BINDING COMPOUND, USE, ANTITUMOUR PHARMACEUTICAL COMPOSITION, GENE CONSTRUCT FOR EXPRESSING AN HSP70 BINDER, PRODUCTION PROCESS, PROCESS FOR EVALUATING TUMOUR CELLS, METHOD OF SENSITIZATION OF TUMOUR CELLS TO CHEMOTHERAPEUTIC DRUGS

HSP70 BINDING COMPOUND, USE, ANTITUMOUR PHARMACEUTICAL COMPOSITION, GENE CONSTRUCT FOR EXPRESSING AN HSP70 BINDER, PRODUCTION PROCESS, PROCESS FOR EVALUATING TUMOUR CELLS, METHOD OF SENSITIZATION OF TUMOUR CELLS TO CHEMOTHERAPEUTIC DRUGS

机译：HSP70结合剂，用途，抗药性成分，表达HSP70结合剂的基因结构，生产过程，评估肿瘤细胞的过程，将肿瘤细胞敏化为化疗药物的方法

页面导航

摘要
著录项
相似文献

摘要

The present invention provides the use of the HspBP1 protein or portion thereof in preparing an antitumour pharmaceutical composition, the combination thereof with one or more chemotherapeutic agents being optional. The following are also disclosed: a gene construct for expressing said binder, a production process, a process for evaluating tumour cells, and a method of sensitization of tumours to chemotherapeutic drugs. The invention also provides a novel in vivo and in vitro experimental model for developing/evaluating antitumour agents. The pharmaceutical compositions of the invention provide antitumour activity and are highly specific for neoplastic cells, resulting in less cytotoxicity for cells with normal cell division. These and other advantages of the invention characterize a solution for the inconveniences of the currently known chemotherapeutic treatments.

机译：本发明提供了HspBP1蛋白或其部分在制备抗肿瘤药物组合物中的用途，其与一种或多种化学治疗剂的组合是任选的。还公开了以下内容：用于表达所述结合剂的基因构建体，生产方法，评估肿瘤细胞的方法以及使肿瘤对化学治疗药物敏感的方法。本发明还提供了用于开发/评估抗肿瘤剂的新颖的体内和体外实验模型。本发明的药物组合物提供抗肿瘤活性并且对肿瘤细胞具有高度特异性，从而导致具有正常细胞分裂的细胞的细胞毒性较小。本发明的这些和其他优点是解决目前已知的化学治疗方法的不便的解决方案。 展开▼

著录项

公开/公告号WO2014100883A1

专利类型

公开/公告日2014-07-03

原文格式PDF

申请/专利权人 UNIÃO BRASILEIRA DE EDUCAÇÃO E ASSISTÊNCIA MANTENEDORA DA PUCRS;
展开▼

申请/专利号WO2013BR00612

发明设计人 CAZABUENA BONORINO CRISTINA BEATRIZ;DUARTE DE SOUZA ANA PAULA;
展开▼

申请日2013-12-27

分类号C07K14/47;A61K38/16;

国家 WO

入库时间 2022-08-21 15:48:47

相似文献

专利

外文文献

中文文献

1. 采用荧光RT-PCR技术检测周氏啮小蜂Hsp70基因表达的方法 [P] . 中国专利： CN105296480B . 2018.10.26

2. 采用荧光RT-PCR技术检测周氏啮小蜂Hsp70基因表达的方法 [P] . 中国专利： CN105296480A . 2016-02-03

3. HSP70 BINDING COMPOUND, USE, ANTITUMOUR PHARMACEUTICAL COMPOSITION, GENE CONSTRUCT FOR EXPRESSING AN HSP70 BINDER, PRODUCTION PROCESS, PROCESS FOR EVALUATING TUMOUR CELLS, METHOD OF SENSITIZATION OF TUMOUR CELLS TO CHEMOTHERAPEUTIC DRUGS [P] . 欧洲知识产权局专利： EP2940040A4 . 2016-08-03

机译： HSP70结合剂，用途，抗药性成分，表达HSP70结合剂的基因结构，生产过程，评估肿瘤细胞的过程，将肿瘤细胞敏化为化疗药物的方法

4. HSP70 BINDING COMPOUND, USE, ANTITUMOUR PHARMACEUTICAL COMPOSITION, GENE CONSTRUCT FOR EXPRESSING AN HSP70 BINDER, PRODUCTION PROCESS, PROCESS FOR EVALUATING TUMOUR CELLS, METHOD OF SENSITIZATION OF TUMOUR CELLS TO CHEMOTHERAPEUTIC DRUGS [P] . 欧洲知识产权局专利： EP2940040A1 . 2015-11-04

机译： HSP70结合剂，用途，抗药性成分，表达HSP70结合剂的基因结构，生产过程，评估肿瘤细胞的过程，将肿瘤细胞敏化为化疗药物的方法

5. Compound, use, anti-tumor pharmaceutical composition, gene construct for polypeptide expression, process for the production, process for the evaluation of tumor cells, method of sensitization of tumor cells to chemotherapeutic [P] . 美国专利： US10508139B2 . 2019-12-17

机译：化合物，用途，抗肿瘤药物组合物，用于多肽表达的基因构建体，生产方法，评估肿瘤细胞的方法，肿瘤细胞对化学疗法的致敏方法

1. Three Dimensional Simulation Method in Early Process of Division and Growth for Tumour Cells [J] . XIA Zhi-qiu, ZHAO Ting-ting 计算机辅助绘图设计与制造（英文版） . 2014,第003期

2. Three Dimensional Simulation Method in Early Process of Division and Growth for Tumour Cells [J] . XIA Zhi-qiu, ZHA O Ting-ting 计算机辅助绘图．设计与制造：英文版 . 2014,第003期

3. Differences in phenotype and gene expression of prostate stromal cells from patients of varying ages and their influence on tumour formation by prostate epithelial cells [J] . Yong-Chuan Wang, Sheng-Qiang Yu, Xiao-Hai Wang, 亚洲男性学杂志（英文版） . 2011,第005期

4. Membrane-bound HSP70-engineered myeloma cell-derived exosomes stimulate more efficient CD8(+) CTL- and NK-mediated antitumour immunity than exosomes released from heat-shocked tumour cells expressing cytoplasmic HSP70. [J] . Xie Y, Bai O, Zhang H, Journal of cellular and molecular medicine. . 2010,第11期

5. Membrane-bound HSP70-engineered myeloma cell-derived exosomes stimulate more efficient CD8⁺ CTL- and NK-mediated antitumour immunity than exosomes released from heat-shocked tumour cells expressing cytoplasmic HSP70 [J] . Yufeng Xie, Ou Bai, Haifeng Zhang, Journal of cellular and molecular medicine. . 2010,第11期

6. 256 Structural mimics of heat shock protein70 (Hsp70) associated peptides from breast tumour cells can prime T cells to respond to tumour antigens [J] . B.Arnaiz, L.Madrigal, D.Doherty, European Journal of Cancer Supplements . 2004,第8期

7. DYSREGULATION OF THE RAS-ONCOGENE AND P53 TUMOUR SUPPESSOR GENE EXPRESSION IN THE POLYURETHANE INDUCED TUMOUR CELL LINES IN VITRO [C] . Tingfei Xi, Chunren Wang, Qing Zhang, The 6th International Conference on Biomedical Engineering and Rehabilitation Engineering (ICBME '2002) May 27-30, 2002 Guilin, China . 2002

8. Evaluation of Agrobacteria-mediated Transient Expression of Cell Wall Degrading Enzymes as a Biological Pretreatment Process for Cellulosic Biofuel Production. [D] . Anthony, Elizabeth Anne. 2016

9. Membrane-bound HSP70-engineered myeloma cell-derived exosomes stimulate more efficient CD8+ CTL- and NK-mediated antitumour immunity than exosomes released from heat-shocked tumour cells expressing cytoplasmic HSP70 [O] . Yufeng Xie, Ou Bai, Haifeng Zhang, 2010

10. Poster session 2Morphogenetic mechanisms290MiR-133 regulates retinoic acid pathway during early cardiac chamber specification291Bmp2 regulates atrial differentiation through miR-130 during early heart looping formationDevelopmental genetics294Association of deletion allele of insertion/deletion polymorphism in alpha 2B adrenoceptor gene and hypertension with or without type 2 diabetes mellitus295Association of G1359A polymorphism of the endocannabinoid type 1 receptor (CNR1) with coronary artery disease (CAD) with type 2 diabetes mellitusCell growth, differentiation and stem cells - Vascular298Gamma-secretase inhibitor prevents proliferation and migration of ductus arteriosus smooth muscle cells: a role of Notch signaling in postnatal closure of ductus arteriosus299Mesenchymal stromal-like cells (MLCs) derived from induced pluripotent stem (iPS) cells: a promising therapeutic option to promote neovascularization300Sonic Hedgehog promotes mesenchymal stem cell differentiation to vascular smooth muscle cells in cardiovacsular disease301Proinflammatory cytokine secretion and epigenetic modification in endothelial cells treated LPS-GinfivalisCell death and apoptosis - Vascular304Mitophagy acts as a safeguard mechanism against human vascular smooth muscle cell apoptosis induced by atherogenic lipidsTranscriptional control and RNA species - Vascular307MicroRNA-34a role in vascular calcification308Local delivery of a miR-146a inhibitor utilizing a clinically applicable approach attenuates neointima formation after vascular injury309Long noncoding RNA landscape of hypoxic endothelial cells310Specific circulating microRNAs levels associate with hypertension, hyperglycemia and dysfunctional HDL in acute coronary syndrome patientsCytokines and cellular inflammation - Vascular313Phosphodiesterase5A up-regulation in vascular endothelium under pro-inflammatory conditions: a newly disclosed anti-inflammatory activity for the omega-3polyunsaturated aatty acid docosahexaenoic acid314Cardiovascular risk modifying with extra-low dose anticytokine drugs in rhematoid arthritis315Conversion of human M-CSF macrophages into foam cells reduces their proinflammatory responses to classical M1-polarizing activation316Lymphocytic myocarditis coincides with increased plaque inflammation and plaque hemorrhage in coronary arteries, facilitating myocardial infarction317Serum osteoprotegerin level predictsdeclined numerous of circulating endothelial- derived and mononuclear-derived progenitor cells in patients with metabolic syndromeGrowth factors and neurohormones - Vascular320Effect of gastrin-releasing peptide (GRP) on vascular inflammationSignal transduction - Heart323A new synthetic peptide regulates hypertrophy in vitro through means of the inhibition of nfkb324Inducible fibroblast-specific knockout of p38 alpha map kinase is cardioprotective in a mouse model of isoproterenol-induced cardiac hypertrophy325Regulation of beta-adrenoceptor-evoked inotropic responses by inhibitory G protein, adenylyl cyclase isoforms 5 and 6 and phosphodiesterases326Binding to RGS3 and stimulation of M2 muscarinic acetylcholine receptors modulates the substrate specificity of p190RhoGAP in cardiac myocytes327Cardiac regulation of post-translational modifications, parylation and deacetylation in LMNA dilated cardiomyopathy mouse model328Beta-adrenergic regulation of the b56delta/pp2a holoenzyme in cardiac myocytes through b56delta phosphorylation at serine 573Nitric oxide and reactive oxygen species - Vascular331Oxidative stress-induced miR-200c disrupts the regulatory loop among SIRT1, FOXO1 and eNOS332Antioxidant therapy prevents oxidative stress-induced endothelial dysfunction and Enhances Wound Healing333Morphological and biochemical characterization of red blood cell in coronary artery diseaseCytoskeleton and mechanotransduction - Heart336Novel myosin activator, JSH compounds, increased myocardial contractility without chronotropic effect in ratsExtracellular matrix and fibrosis - Vascular339Ablation of Toll-like receptor 9 causes cardiac rupture after myocardial infarction by attenuating proliferation and differentiation of cardiac fibroblasts340Altered vascular remodeling in the mouse hind limb ischemia model in Factor VII activating protease (FSAP) deficiencyVasculogenesis, angiogenesis and arteriogenesis343Pro-angiogenic effects of proly-hydroxylase inhibitors and their potential for use in a novel strategy of therapeutic angiogenesis for coronary total occlusion344Nrf2 drives angiogenesis in transcription-independent manner: new function of the master regulator of oxidative stress response345Angiogenic gene therapy, despite efficient vascular growth, is not able to improve muscle function in normoxic or chronically ischemic rabbit hindlimbs -role of capillary arterialization and shunting346Effect of PAR-1 inhibition on collateral vessel growth in the murine hind limb model347Quaking is a key regulator of endothelial cell differentiation, neovascularization and angiogenesis348"Emerging angiogenesis" in the chick chorioallantoic membrane (CAM). An in vivo study349Exosomes from cardiomyocyte progenitor cells and mesenchymal stem cells stimulate angiogenesis in vitro and in vivo via EMMPRINEndothelium352Reciprocal regulation of GRK2 and bradykinin receptor stimulation modulate Ca2+ intracellular level in endothelial cells353The roles of bone morphogenetic proteins 9 and 10 in endothelial inflammation and atherosclerosis354The contribution of GPR55 to the L-alpha-lysophosphatidylinositol-induced vasorelaxation in isolated human pulmonary arteries355The endothelial protective ACE inhibitor Zofenoprilat exerts anti-inflammatory activities through H2S production356A new class of glycomimetic drugs to prevent free fatty acid-induced endothelial dysfunction357Endothelial progenitor cells to apoptotic endothelial cell-derived microparticles ration differentiatesas preserved from reduced ejection fractionheart failure358Proosteogenic genes are activated in endothelial cells of patients with thoracic aortic aneurysm359Endothelin ETB receptors mediate relaxing responses to insulin in pericardial resistance arteries from patients with cardiovascular disease (CVD)Smooth muscle and pericytes362CX3CR1 positive myeloid cells regulate vascular smooth muscle tone by inducing calcium oscillations via activation of IP3 receptors363A novel function of PI3Kg on cAMP regulation, role in arterial wall hyperplasia through modulation of smooth muscle cells proliferation364NRP1 and NRP2 play important roles in the development of neointimal hyperplasia in vivo365Azithromycin induces autophagy in aortic smooth muscle cellsCoagulation, thrombosis and platelets368The real time in vivo evaluation of platelet-dependent aldosterone prothrombotic action in mice369Development of a method for in vivo detection of active thrombi in mice370The antiplatelet effects of structural analogs of the taurine chloramine371The influence of heparin anticoagulant drugs on functional state of human platelets372Regulation of platelet aggregation and adenosine diphosphate release by d dimer in acute coronary syndrome (in vitro study)Oxygen sensing, ischaemia and reperfusion375Sirtuin 5 mediates brain injury in a mouse model of cerebral ischemia-reperfusion376Abscisic acid: a new player in cardiomyocyte protection from ischaemia?377Protective effects of ultramicronized palmitoylethanolamide (PEA-um) in myocardial ischaemia and reperfusion injury in vivo378Identification of stem cell-derived cardiomyocytes using cardiac specific markers and additional testing of these cells in simulated ischemia/reperfusion system379Single-dose intravenous metformin treatment could afford significant protection of the injured rat kidney in an experimental model of ischemia-reperfusion380Cardiotoxicity of long acting muscarinic receptor antagonists used for chronic obstructive pulmonary disease381Dependence antioxidant potential on the concentration of amino acids382The impact of ischemia-reperfusion on physiological parameters,apoptosis and ultrastructure of rabbit myocardium with experimental aterosclerosisMitochondria and energetics385MicroRNA-1 dependent regulation of mitochondrial calcium uniporter (MCU) in normal and hypertrophied hearts386Mitochondrial homeostasis and cardioprotection: common targets for desmin and aB-crystallin387Overexpression of mitofusin-2 (Mfn2) and associated mitochondrial dysfunction in the diabetic heart388NO-dependent prevention of permeability transition pore (MPTP) opening by H2S and its regulation of Ca2+ accumulation in rat heart mitochondria389G protein coupled receptor kinase 2 (GRK2) is fundamental in recovering mitochondrial morphology and function after exposure to ionizing radiation (IR)Gender issues392Sex differences in pulmonary vascular control; focus on the nitric oxide pathwayAging395Heart failure with preserved ejection fraction develops when feeding western diet to senescence-accelerated mice396Cardiovascular markers as predictors of cognitive decline in elderly hypertensive patients397Changes in connexin43 in old rats with volume overload chronic heart failureGenetics and epigenetics400Calcium content in the aortic valve is associated with 1G>2G matrix metalloproteinase 1 polymorphism401Neuropeptide receptor gene s (NPSR1) polymorphism and sleep disturbances402Endothelin-1 gene Lys198Asn polymorphism in men with essential hypertension complicated and uncomplicated with chronic heart failure403Association of common polymorphisms of the lipoprotein lipase and pon1 genes with the metabolic syndrome in a sample of community participantsGenomics, proteomics, metabolomics, lipidomics and glycomics405Gene expression quantification using multiplexed color-coded probe pairs to determine RNA content in sporadic cardiac myxoma406Large-scale phosphorylation study of the type 2 diabetic heart subjected to ischemia / reperfusion injury407Transcriptome-based identification of new anti-inflammatory properties of the olive oil hydroxytyrosol in vascular endothelial cell under basal and proinflammatory conditions408Gene polymorphisms combinations and risk of myocardial infarctionComputer modelling, bioinformatics and big data411Comparison of the repolarization reserve in three state-of-the-art models of the human ventricular action potentialMetabolism, diabetes mellitus and obesity414Endothelial monocyte-activating polypeptide-II improves heart function in type -I Diabetes mellitus415Admission glucose level is independent predictor of impaired left ventricular function in patients with acute myocardial infarction: a two dimensional speckle-tracking echocardiography study416Association between biochemical markers of lipid profile and inflammatory reaction and stiffness of the vascular wall in hypertensive patients with abdominal obesity417Multiple common co-morbidities produce left ventricular diastolic dysfunction associated with coronary microvascular dysfunction, oxidative stress and myocardial stiffening418Investigating the cardiovascular effects of antiretroviral drugs in a lean and high fat/sucrose diet rat model of obesity419Statins in the treatment of non-alcoholic steatohepatitis (NASH). Our experience from a 2-year prospective study in Constanta County, Romania420Epicardial adipose tissue as a predictor of cardiovascular outcome in patients with ACS undergoing PCI?Arterial and pulmonary hypertension423Dependence between heart rhythm disorers and ID polymorphism of ACE gene in hypertensive patients424Molecular mechanisms underlying the beneficial effects of Urocortin 2 in pulmonary arterial hypertension425Inhibition of TGf-b axis and action of renin-angiotensin system in human ascending aorta aneurysms426Early signs of microcirculation and macrocirculation abnormalities in prehypertension427Vascular smooth muscle cell-expressed Tie-2 controls vascular tone428Cardiac and vascular remodelling in the development of chronic thrombo-embolic pulmonary hypertension in a novel swine modelBiomarkers431Arrhythmogenic cardiomyopathy: a new, non invasive biomarker432Can circulating microRNAs distinguish type 1 and type 2 myocardial infarction?433Design of a high-throughput multiplex proteomics assay to identify left ventricular diastolic dysfunction in diabetes434Monocyte-derived and P-selectin-carrying microparticles are differently modified by a low fat diet in patients with cardiovascular risk factors who will and who will not develop a cardiovascular event435Red blood cell distribution width assessment by polychromatic interference microscopy of thin films in chronic heart failure436Invasive and noninvasive evaluation of quality of radiofrequency-induced cardiac denervation in patients with atrial fibrillation437The effect of therapeutic hypothermia on the level of brain derived neurotrophic factor (BDNF) in sera following cardiopulmonary resustitation438Novel biomarkers to predict outcome in patients with heart failure and severe aortic stenosis439Biological factors linking depression and anxiety to cardiovascular disease440Troponins and myoglobin dynamic at coronary arteries graftingInvasive, non-invasive and molecular imaging443Diet composition effects on the genetic typing of the mouse ob mutation: a micro-ultrasound characterization of cardiac function, macro and micro circulation and liver steatosis444Characterization of pig coronary and rabbit aortic lesions using IV-OCT quantitative analysis: correlations with histologyGene therapy and cell therapy447Enhancing the survival and angiogenic potential of mouse atrial mesenchymal cells448VCAM-1 expression in experimental myocardial infarction and its relation to bone marrow-derived mononuclear cell retentionTissue engineering451Advanced multi layered scaffold that increases the maturity of stem cell-derived human cardiomyocytes452Response of engineered heart tissue to simulated ischemia/reperfusion in the presence of acute hyperglycemic conditions453Serum albumin hydrogels prevent de-differentiation of neonatal cardiomyocytes454A novel paintbrush technique for transfer of low viscosity ultraviolet light curable cyan methacrylate on saline immersed in-vitro sheep heart [O] . V Garcia-Martinez, C Lopez Sanchez, W Hamed, 2016

1. Transduction of Fas gene or Bcl-2 antisense RNA sensitizes cultured drug resistant gastric cancer cells to chemotherapeutic drugs [J] . XIAO Bing SHI Yong Quan ZHAO Yan Qiu YOU Han WANG Zuo You LIU Xian Ling YIN Fang QIAO Tai Dong and FAN Dai Ming . 世界胃肠病学杂志：英文版 . 1998,第5期

2. Interaction between the Wilms tumour factor-1 element in the promoter of Amh and a downstream enhancer is required for a strong expression of the gene in pre-pubertal sertoli cells [J] . David W. Dresser . 美国分子生物学期刊（英文） . 2013,第3期

3. 基因重组人HSP73抗体的制备及对肿瘤细胞在温热作用后HSP70表达的检测 [C] . 杨虎川 ,杨耀琴 ,陈斌 . 第四届全路肿瘤学术会议 . 2000

4. 高表达HSP70的肿瘤细胞裂解液对结肠癌小鼠抗肿瘤机制研究 [A] . 申艳梅 . 2012