首页> 外国专利> SEQUENCING DEVICE FOR SEQUENCING AT LEAST ONE INDIVIDUAL NUCLEIC ACID STRAND, AND METHOD FOR SEQUENCING AT LEAST ONE INDIVIDUAL NUCLEIC ACID STRAND

SEQUENCING DEVICE FOR SEQUENCING AT LEAST ONE INDIVIDUAL NUCLEIC ACID STRAND, AND METHOD FOR SEQUENCING AT LEAST ONE INDIVIDUAL NUCLEIC ACID STRAND

机译:用于至少一个单独的核酸链的测序的装置和用于至少一个单独的核酸链的测序的方法

摘要

The invention relates to a sequencing device for sequencing at least one individual nucleic acid strand (10), comprising a sample retainer (12) on which a conductive surface (14) with at least one polymerase (16) is secured or can be secured. The sequencing device additionally comprises a nucleotide feed device (22), by means of which different types of nucleotides (24) can be fed onto the conductive surface (14) in a specifiable sequence, an excitation device (28), by means of which an alternating electric field can be generated, a detection device (36), by means of which at least one varying signal (38) can be ascertained, and an analyzing device (40) designed to output information (44) relating to a type of a nucleotide (24) that is polymerized onto at least one primer (18) and/or the complementary nucleotide of at least one individual nucleic acid strand (10) while taking into consideration the at least one varying signal (38). The invention also relates to a method for sequencing at least one individual nucleic acid strand (10).
机译:本发明涉及一种用于对至少一条单独的核酸链(10)进行测序的测序装置,其包括样品保持器(12),在其上固定或可以固定具有至少一种聚合酶(16)的导电表面(14)。测序装置另外包括核苷酸进料装置(22),通过其可以将不同类型的核苷酸(24)以可指定的顺序进料到导电表面(14)上,通过激发装置(28)。可以产生一个交变电场,一个检测装置(36),通过它可以确定至少一个变化的信号(38),以及一个分析装置(40),该分析装置被设计为输出与以下类型有关的信息(44)。考虑到至少一种变化的信号(38)时,其被聚合到至少一条引物(18)和/或至少一条单独的核酸链(10)的互补核苷酸上的核苷酸(24)。本发明还涉及一种用于对至少一条单独的核酸链(10)进行测序的方法。

著录项

  • 公开/公告号WO2014127971A1

    专利类型

  • 公开/公告日2014-08-28

    原文格式PDF

  • 申请/专利权人 SIEMENS AKTIENGESELLSCHAFT;

    申请/专利号WO2014EP51887

  • 发明设计人 HAYDEN OLIVER;WARNAT GERALD;

    申请日2014-01-31

  • 分类号C12Q1/68;

  • 国家 WO

  • 入库时间 2022-08-21 15:47:51

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