MANUFACTURING METHOD FOR GRAPHENE BASED HIGH EFFICIENCY FIELD-EFFECT TRANSISTOR CANCER DIAGNOSING APTASENSOR WHICH IS DOPED WITH NITROGEN MANUFACTURED FROM CONDUCTIVE POLYMER THIN FILM APPLYING SCREEN PRINTING TECHNIQUE METHOD FOR MANUFACTURING HIGH-EFFICIENCY FIELD-EFFECT TRANSISTOR TYPE APTASENSOR FOR CANCER DIAGNOSIS BASED ON GRAPHENE DOPED WITH NITROGEN MANUFACTURED FROM CONDUCTIVE POLYMER THIN FILM USING SCREEN PRINTING TECHNIQUE

摘要

The present invention relates to a method for manufacturing a field-effect transistor type aptasensor using graphene doped with nitrogen having aptamer. The disclosed method is to detect an angiogenesis factor by transferring nitrogen doped graphene having excellent electrical properties manufactured from a conductive polymer onto a glass substrate, by fixating an antineoplastic angiogenesis factor aptamer having high affinity with the angiogenesis factor to the graphene through covalent bonding, and by monitoring current changes inside a liquid-ion gate field-effect transistor array in real time. According to the present invention, the disclosed method can easily manufacture nitrogen doped graphene with high conductivity using a simple and effective screen printing and vapor deposition polymerization. In addition, the manufactured nitrogen doped graphene is transferred to a glass substrate in order to maintain electrical contact through deposition of a metal electrode, and an aptamer can be stably introduced onto the surface of the graphene through chemical bonding. The aptamer can induce the accumulation and reduction of a charge carrier inside the graphene through reaction to a specific target material, and can provide improved sensitivity and real-time reaction because the interaction with an object to be analyzed is increased by the electrical properties of the graphene having high conductivity.;COPYRIGHT KIPO 2014