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DNA markers for Ganoderma lucidum, primers for the markers, and method for discriminating Ganoderma lucidum

机译:灵芝的DNA标记,该标记的引物和鉴别灵芝的方法

摘要

PURPOSE: A molecular marker of ganoderma lucidum is provided to distinguish ganoderma ucidum species by a molecular biological analyzing method, and to expect quality management of imported ganoderma lucidum. CONSTITUTION: A composition for marker of ganoderma lucidum comprises sequence number 1 of base sequence as an active ingredient. A pair of primers having sequence number 2 and 3 is provided. A composition for discriminating ganoderma lucidum comprises the pair of primers as an active ingredient. A distinguishing method of ganoderma lucidum comprises the steps of: separating genome DNA from the mushroom; amplifying a target sequence by performing an amplification using the oligonucleotide primer set, taking the separated genome DNA as a frame; and detecting the amplified product.
机译:目的:提供一种灵芝的分子标记,以通过分子生物学分析方法区分灵芝物种,并期望进口灵芝的质量管理。组成:灵芝标记组合物,包含碱基序列的序列号1作为有效成分。提供一对具有序列号2和3的引物。用于鉴别灵芝的组合物包含一对引物作为活性成分。灵芝的一种鉴别方法包括以下步骤:从蘑菇中分离基因组DNA;以及从蘑菇中分离基因组DNA。通过使用寡核苷酸引物组进行扩增,以分离的基因组DNA为框架,扩增靶序列。并检测扩增产物。

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