Multiplex Microfluidic Device for Screening Nucleic Acid Aptamer and High Throughput Method of Screening Nucleic Acid Aptamer Using the Same

摘要

The present invention relates to a multiplex microfluidic device for selecting nucleic acid aptamers, and a high throughput selection method for nucleic acid aptamers using the same, and more specifically, to a multiplex microfluidic device (SELEX lap-on-a-chip) in which a conventional aptamer development method for a single target is converted into an improved multiplex platform, and a high throughput selection method for nucleic acid aptamers in which the method is linked with high throughput sequencing. According to the present invention, the multiplex microfluidic device can simultaneously detect each aptamer for a plurality of targets and remarkably reduces the processing amount and processing time compared with a conventional multiplex method. In particular, the target coupling/separation/amplification process round can be greatly reduced and an automated process can carry out a selection process of aptamers by linking the device of the present invention with high throughput sequencing, and thus the device is very useful.