A use of mammalian vector including T7 promoter and N-terminal HA tag for the over-expression of human genes in E. coli

摘要

foreign proteins under control of the T7 promoter in the present invention is E. coli does not contain the T7 RNA polymerase , preferably , to a technique for expressing human protein . More particularly, the present invention is the T7 promoter and the N- terminal HA tag purpose using a vector containing the sequence for enhancing expression of foreign proteins in E. coli do not contain the T7 RNA polymerase , including the vector and T7 RNA polymerase E. coli has an enhanced expression of foreign proteins without the need for , or production of the foreign protein expression method using E. coli , and to a functional analysis of the foreign protein . The invention further relates to a method using the vector to simplify the toxicity and expression in cells of the human gene in E. coli and rapid diagnosis . ;