EXPRESSION OF HUMAN RECOMBINANT INTERLEUKIN-1 RECEPTOR ANTAGONIST (rhIL-1ra) IN E. COLI

摘要

Using restriction endonuclease Hind,. Sal i, the IL-lra cDNA was isolated from PRCra, then by a medium plasmid pUC19, It was cloned into a high level expressive vector PBV220. Therecombinant plasmid pBV-IL--Ira waS transrormed Into E. coil DH-So. After induced by temperaIure, a 20KD protein could be detected by SDS-PAGE. Biological assay in vitro showed that the protein could inhibit the activity or IL-l obviously.