GENETIC CONSTRUCTION, WHICH CONTAINS HGDNF UNDER THE CONTROL OF temperature-sensitive PROMOTOR FOR ADJUSTABLE EXPRESSION OF neurotrophin FACTOR BOTH THE B CELLS, AND DIRECTLY B THE ORGANISM OF THE MAMMALS

摘要

The genetic construct expressing GDNF in cells and tissues of mammals (including human) as the temperature increases, constructed on the basis of the vector plasmid pEGFP-N1 with a neomycin resistance gene comprising a DNA fragment encoding a regulatory promoter sequence with elements of heat shock HSE more 4, a protein gene promoter, heat shock hsp70 Drosophila melanogaster, human gene-derived neurotrophic factor GDNF, green fluorescent protein gene (GFP), under the control of a promoter, wherein the promoter is capable of activated by heat or heat shock mammalian toxic effects.