COMPOSITIONS BASED ON STABILIZED FIBRONECTIN DOMAINS, METHODS AND AREAS OF THEIR APPLICATION

摘要

1. A polypeptide containing a framework-based molecule containing loop domains topologically similar to type III fibronectin domains, where the polypeptide has an amino acid sequence based on a consensus sequence of type III fibronectin domains having the amino acid sequence of SEQ ID NO: 16, in which the specific residues of the SEQ sequence ID NO: 16 replaced, which increases the chemical stability and thermal stability of the specified molecule based on the framework. 2. The polypeptide according to claim 1, wherein the thermal stability is measured as the melting temperature (Tm) using differential scanning calorimetry, and the chemical stability is measured as [D] by the stability to the chloride induced guanidine denaturation. The polypeptide according to claim 2, in which the Tm is increased compared to a molecule based on the frame having the amino acid sequence of SEQ ID NO: 16 with an energy value of from about 4.18 kJ (1 kcal) to about 50.21 kJ (12 kcal). four. The polypeptide according to claim 2, in which [D] compared with the frame-based molecule having the amino acid sequence of SEQ ID NO: 16, increases from about 0.1 to about 2.0 M, and free energy (AG) compared to a skeleton-based molecule having the amino acid sequence of SEQ ID NO: 16 increases from 0.418 to about 41.8 kJ / mol (from 0.1 to about 10 kcal / mol) .5. A polypeptide containing a framework-based molecule containing loop domains topologically similar to type III fibronectin domains, where the polypeptide has an amino acid sequence based on a consensus sequence of type III fibronectin domains having an amino acid