COMPOSITIONS BASED ON STABILIZED FIBRONECTIN DOMAINS, METHODS AND AREAS OF APPLICATION THEREOF

摘要

FIELD: chemistry.;SUBSTANCE: invention relates to a method of constructing a library of proteins obtained from a consensus sequence of a domain of fibronectin of type III (FN3) with higher stability and a library obtained using such method. Method involves the following stages: providing a polynucleotide sequence coding a polypeptide containing a consensus sequence of a domain of fibronectin of type III (FN3) with higher stability, selected from a group consisting of SEQ ID NO: 142-151; introduction of randomized codons into the polynucleotide sequence in the selected positions; and reproduction of copies of the said polynucleotide sequence to produce a library of polynucleotides coding versions of scaffold proteins.;EFFECT: inventions can be used in biopharmaceutical industry.;4 cl, 9 dwg, 5 tbl, 4 ex