COMPOSITION, METHODS FOR PRODUCING AND APPLICATION OF A FRAME BASED ON A FIBRONECTIN TYPE III DOMAIN

摘要

1. An isolated protein framework based on the type III fibronectin domain (FN3), comprising an amino acid sequence derived from the consensus sequence of the FN3 domain, wherein the amino acid sequence is at least 75% identical to the consensus sequence of the FN3.2 domain. An isolated protein framework based on the type III fibronectin domain (FN3) containing the amino acid sequence derived from the consensus sequence of the FN3 domain, wherein the amino acid sequence is at least 75% identical to SEQ ID NO: 16.3. The protein framework according to claim 2, containing 7 strands and 6 loops between them. The protein framework according to claim 3, containing the amino acid sequence of SEQ ID NO: 16 and forming loops in the region of residues 13-16, 22-28, 38-43, 51-54, 60-64 and 75-81 of the sequence SEQ ID NO: 16 capable of binding to cellular proteins and / or nucleic acid molecules. 5. The protein framework according to claim 3, containing the amino acid sequence of SEQ ID NO: 16 and forming loops in the region of residues 13-16, 22-28, 38-43, 51-54, 60-64 and 75-81 of the sequence SEQ ID NO: 16 capable of binding to cellular proteins and / or nucleic acid molecules, wherein one or more loops are altered to bind to a target. The protein framework according to any one of claims 1 to 5, wherein said framework connects the target with at least one of affinity values selected from at least 10M, at least 10M, at least 10M, and at least 10M, at least 10M, at least 10M and at least 10M, determined by surface plasmon resonance or kinetic exclusion analysis (Kinexa). 7. The protein framework according to any one of claims 1 to 5, where