VACCINE OF VIRUS-LIKE PARTICLES CONTAINING ALL HEPATITIS C VIRUS STRUCTURAL ANTIGENS, AND METHOD FOR PREPARING IT IN Hansenula polymorpha YEAST

摘要

FIELD: medicine, pharmaceutics.;SUBSTANCE: invention refers to biotechnology and represents a method for preparing virus-like particles containing hepatitis C virus core, E1 and E2 structural antigens, and a method for particle purification. The method for preparing the virus-like particles involves Hansenula polymorpha yeast cell transformation with a recombinant plasmid containing one open reading frame carrying hepatitis C virus core-E1-E2 genes a direct expression of which leads to the formation of core, E1 and E2 antigens. That is followed by culturing and recovering the prepared particles. A method for virus-like particle purification involves bleaching of a damaged cell homogenate, diafiltration, concentration, precipitation with low pH, ion-exchange chromatography, sedimentation centrifugation in a density gradient, hydrophobic chromatography and gel chromatography.;EFFECT: invention enables preparing the high-yield virus-like particles applicable as a vaccine for preventing and/or treating the diseases caused by hepatitis C virus.;2 cl, 5 dwg, 5 ex