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CELL PEELING DEVICE, CELL PEELING METHOD, AND CELL CULTURE SYSTEM

机译:细胞传代装置,细胞传代方法和细胞培养系统

摘要

PROBLEM TO BE SOLVED: To provide a cell peeling device, a cell peeling method, and a cell culture system which are capable of easily peeling adherent cultured cells from a foothold culture medium, such as carrier for proliferation, with a simple constitution.SOLUTION: A cell peeling device comprises: a peeling tank 2; a culture medium supply line 3 which supplies culture medium 14 containing a carrier attached with cells into the peeling tank; a peeling liquid supply line 5 which supplies peeling liquid to the peeling tank; a filter 9 which is provided in a lower part of the peeling tank and has mesh larger than the cells and smaller than the carrier; a waste liquid line 6 connected to the peeling tank below the filter; and a shaking mechanism 8 which oscillates the peeling tank, in which the peeling liquid 12 is supplied from the peeling liquid supply line into the peeling tank from which the culture medium is discharged via the waste liquid line, the shaking mechanism oscillates the peeling tank, and allows the carrier to flow to peel the cells.
机译:要解决的问题:提供一种细胞剥离装置,细胞剥离方法和细胞培养系统,它们能够以简单的结构容易地从立足培养基(例如用于增殖的载体)中剥离附着的培养细胞,以解决上述问题。细胞剥离装置包括:剥离槽2;和剥离槽2。培养基供给线3,其将含有附着有细胞的载体的培养基14供给到剥离槽中。剥离液供给管线5,其向剥离罐供给剥离液。过滤器9,其设置在剥离槽的下部,并且具有比小孔大且小于载体的筛孔。连接至过滤器下方的剥离罐的废液管线6;振荡机构8,其使剥离槽振荡,其中,剥离液12从剥离液供给管线被供给到经由废液管线排出培养基的剥离槽,该振荡机构使剥离槽振荡,并允许载体流动以剥离细胞。

著录项

  • 公开/公告号JP2015123060A

    专利类型

  • 公开/公告日2015-07-06

    原文格式PDF

  • 申请/专利权人 IHI CORP;

    申请/专利号JP20130271603

  • 发明设计人 MORI MIE;ISHII KOSUKE;

    申请日2013-12-27

  • 分类号C12M1;

  • 国家 JP

  • 入库时间 2022-08-21 15:32:54

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