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METHOD OF DETERMINING CELL PEELING, METHOD OF PEELING CULTURED CELLS, DEVICE FOR DETERMINING CELL PEELING AND AUTOMATED CELL CULTURE APPARATUS
METHOD OF DETERMINING CELL PEELING, METHOD OF PEELING CULTURED CELLS, DEVICE FOR DETERMINING CELL PEELING AND AUTOMATED CELL CULTURE APPARATUS
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机译:测定细胞上皮的方法,上皮细胞的上皮方法,测定上皮细胞的设备和自动细胞培养装置
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摘要
A novel method for evaluating cell detachment which can be used in passage operation and the like in culture of adhesive cells; a method of detaching a cultured cell utilizing the same; an apparatus for evaluating cell detachment for carrying out the aforementioned method for evaluating cell detachment; and an automated cell culture apparatus in which the apparatus is used are provided. A first image is taken by imaging a certain position in a culture vessel; a second image is taken by imaging the certain position again after taking the first image and also after allowing the culture liquid in the culture vessel to flow; and the cell detachment from the culture vessel is evaluated based on a cell area ratio A, a cell area ratio B, and a common area ratio C following determination of the common area ratio C for a section where the cell is present in both the first image and the second image based on the cell area ratio A for a section where the cell is present in the first image, and the cell area ratio B for a section where the cell is present in the second image.
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