METHOD OF DETERMINING CELL PEELING, METHOD OF PEELING CULTURED CELLS, DEVICE FOR DETERMINING CELL PEELING AND AUTOMATED CELL CULTURE APPARATUS

摘要

A novel method for evaluating cell detachment which can be used in passage operation and the like in culture of adhesive cells; a method of detaching a cultured cell utilizing the same; an apparatus for evaluating cell detachment for carrying out the aforementioned method for evaluating cell detachment; and an automated cell culture apparatus in which the apparatus is used are provided.;A first image is taken by imaging a certain position in a culture vessel; a second image is taken by imaging the certain position again after taking the first image and also after allowing the culture liquid in the culture vessel to flow; and the cell detachment from the culture vessel is evaluated based on a cell area ratio A, a cell area ratio B, and a common area ratio C following determination of the common area ratio C for a section where the cell is present in both the first image and the second image based on the cell area ratio A for a section where the cell is present in the first image, and the cell area ratio B for a section where the cell is present in the second image.