Method for preparing a mixture of the homodimer protein by using a repulsive interaction of charge

摘要

The present invention, for the preparation of a homodimer protein mixture by using a repulsive interaction of charge How about. The method, different proteins or antibodies is difficult to form a heterodimer with repulsive interactions between similar charge, whereas as same protein or antibody is likely to form a homodimer by attractive interactions between the opposite charges, residue Some of comprising the step of substituting with the oppositely charged residues. Homodimer protein mixtures obtained by the process of the present invention, at the same time acts to different epitopes of the same target, by binding to antigens from different sources, since it is possible that the inhibitory effect of multiple antigens simultaneously, New approaches to immunological diagnosis and therapy of tumors and other diseases is provided.