light analysis device using a single light-emitting particle detection, light analysis method and an optical analysis computer program

摘要

To confocal microscope or multi-photon scanning molecular counting method using an optical measurement by the microscope The Hey, the variation of the detection result of the optical signal of the luminescent particles is suppressed smaller, light analysis techniques for detecting light of the luminescent particles in a sample solution which accuracy can be achieved is provided. Optical analysis technique according to the invention is moved over at least two circumferential and along the position of the optical detection region of the optical system to a predetermined path is placed in the sample solution by changing the optical path of the optical system of the microscope, a plurality of times It generates light intensity data of the time series by detecting the light from the light detection area during the movement of the position of the light sensing region along the predetermined path to be over, circling along the predetermined path of the plurality of photodetecting regions wherein the detecting individually a signal representative of the light from each light emitting particles present in a given path by using the light intensity data of the time series obtained by placing the mobile.