Light analysis device using a single light-emitting particle detection, light analysis method and an optical analysis computer program

摘要

And In the confocal microscope or multi-photon scanning molecule counting method using an optical measurement by microscope, a technique which allows identification or identification of the type of luminescent particles corresponding to the pulse-like signal. The optical analytical techniques for detecting the light of the luminescent particles in a sample solution according to the present invention is placed in the sample solution periodically moved along the position of the optical detection region a predetermined path, the light from the light detection area It generates light intensity data and intensity is measured to detect separately the optical signals of the luminescent particles in the light intensity data. Then, an integer multiple of the movement cycle of the position of the intensity values ​​and the time series by the optical intensity data is measured from the time of occurrence of the optical signals of the one light-emitting particles in the photodetection region of the light signals of one light-emitting particles Based on the intensity values ​​in time separated by the time the corresponding region index value representing the translational diffusion characteristics of the in-emitting particles in a plane perpendicular to the moving direction of the light detection region is determined, by the index value, Identification of the luminescent particles is possible.