Surface plasmon excitation fluorescence measurement device and the surface plasmon excitation fluorescence measurement method .

摘要

PROBLEM TO BE SOLVED: To provide a surface plasmon excitation fluorescence measurement apparatus and a surface plasmon excitation fluorescence measurement method, improving measurement accuracy by reducing the influence of the thickness of a conductor film.;SOLUTION: A surface plasmon excitation fluorescence measurement includes: supplying a sample to a first principal surface of a gold film; fixing an antibody solid layer film to the first principal surface of the gold film; closely contacting a second principal surface of the gold film with a close-contact surface of a prism; fluorescence-labelling an object to be measured captured by the antibody solid layer film; measuring light quantity of surface plasmon excitation fluorescence emitted from the fluorescence-labelled object to be measured when the prism is irradiated with excitation light; measuring light quantity of transmitted light passing through the gold film when the prism is irradiated with film thickness measuring light; and correcting a measurement value of the light quantity of the surface plasmon excitation fluorescence by using a measurement value of the light quantity of transmitted light. In the method, an incident angle θ1 of the excitation light to the close-contact surface is set to a resonance angle θr, and an incident angle θ2 of the film thickness measuring light to the close-contact surface is set to an angle smaller than a critical angle θc.;COPYRIGHT: (C)2013,JPO&INPIT