Technology for inhibiting translation reaction by cleaving part of the poly (A) chain and / or 3 'terminal sequence of mRNA

摘要

Provided is an artificial gene expression suppression method that is easier to design than conventional methods (RNAi method, ribozyme method, and antisense method) and that can easily confirm the effect. A method for suppressing a translation reaction of a target gene, comprising cleaving a part of a poly (A) chain and / or a 3 'terminal sequence of a target mRNA. Kit for suppressing translation reaction comprising a reagent capable of cleaving a part of poly (A) strand and / or 3 ′ terminal sequence of target mRNA; poly (A) strand of target mRNA and / or 3 ′ A cell in which a reagent capable of cleaving a terminal sequence is introduced and the translation reaction of the target gene is suppressed; and the poly (A) strand of the target mRNA and / or a part of the 3 ′ terminal sequence is cleaved And a non-human organism in which the translation reaction of the target gene is suppressed.