Technology for inhibiting translation reaction by cleaving part of the poly (A) chain and / or 3 'terminal sequence of mRNA

摘要

The present invention provides a method of artificially repressing gene expression, which is simpler to design than conventional methods (the RNAi, ribozyme and antisense methods) and which allows for easier confirmation of the effect. A method of inhibiting the translation reaction of a target gene, comprising cutting out a part of the poly(A) tail and/or 3'-terminal sequence of the target mRNA is provided. Also provided are a kit for inhibiting the translation reaction of a target gene, comprising a reagent capable of cutting out a part of the poly(A) tail and/or 3'-terminal sequence of the target mRNA; a cell in which the translation reaction of a target gene is inhibited by introduction thereinto of a reagent capable of cutting out a part of the poly(A) tail and/or 3'-terminal sequence of the target mRNA; and a non-human organism in which the translation reaction of a target gene is inhibited by introduction thereinto of a reagent capable of cutting out a part of the poly(A) tail and/or 3'-terminal sequence of the target mRNA.