ENZYMATIC PREPARATION OF 10 BASE TO 50 KB DOUBLE-STRAND DNA REAGENT FOR SEQUENCING WITH A NANOPORE-POLYMERASE SEQUENCING DEVICE

摘要

The invention herein disclosed provides for devices, reagents, and methods that can detect and control an individual polymer in a mixture is acted upon by another compound, for example, an enzyme, in a nanopore. Of particular note is the use of reagents to rapidly sequence a polynucleotide. The invention is of particular use in the fields of forensic biology, molecular biology, structural biology, cell biology, molecular switches, molecular circuits, and molecular computational devices, and the manufacture thereof.