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Method for quantification of remnant-like lipoprotein cholesterol and kit for same

机译:残留样脂蛋白胆固醇的定量方法及其试剂盒

摘要

A method for quantifying remnant-like lipoprotein cholesterol in a sample simply and accurately without requiring separation operations, and a kit therefor are disclosed. A method for quantifying cholesterol in a remnant-like lipoprotein in a sample containing different lipoproteins including the remnant-like lipoprotein comprises a step (1) of erasing cholesterol in lipoproteins other than the remnant-like lipoprotein; and a step (2) of quantifying cholesterol in the remaining remnant-like lipoprotein. The step (1) is carried out under an action of a cholesterol esterase having a molecular weight of more than 40 kDa and not having a subunit having a molecular weight of not more than 40 kDa; and the step (2) is carried out under an action of a cholesterol esterase having a molecular weight of not more than 40 kDa or a cholesterol esterase having a subunit having a molecular weight of not more than 40 kDa.
机译:公开了一种无需分离操作即可简单,准确地定量样品中残留类脂蛋白胆固醇的方法及其试剂盒。定量包含不同的脂蛋白的样品中的残留样脂蛋白中的胆固醇的方法包括:(1)除去残留样脂蛋白以外的脂蛋白中的胆固醇。步骤(2),定量剩余的残留样脂蛋白中的胆固醇。所述步骤(1)在分子量大于40kDa且不具有分子量不大于40kDa的亚基的胆固醇酯酶的作用下进行; (2)是在分子量为40kDa以下的胆固醇酯酶或分子量为40kDa以下的亚基的胆固醇酯酶的作用下进行的。

著录项

  • 公开/公告号US8906638B2

    专利类型

  • 公开/公告日2014-12-09

    原文格式PDF

  • 申请/专利权人 YUHKO HIRAO;

    申请/专利号US201113884475

  • 发明设计人 YUHKO HIRAO;

    申请日2011-11-09

  • 分类号C12Q1/60;G01N33/92;C12Q1/44;

  • 国家 US

  • 入库时间 2022-08-21 15:16:41

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