Biofunctionalization methodology and characterization of carbon and cobalt-based magnetic nanocomposites for detection of pathogenic microorganisms

摘要

Biofunctionalization methodology and characterization of carbon and cobalt-based magnetic nanocomposites for detection of pathogenic microorganisms. The present invention relates to the methodology for preparing carbon and cobalt (ncm-c / co) magnetic nanocomposites for detection of pathogenic microorganisms. In particular, the methodology described here is based on the adsorption of biological molecules (proteins and antibodies) on the surface of ncm-c / co and the ability of the complex formed (ncm-c / co and biological molecules) to capture by immunomagnetic separation. (yes), pathogenic leptospires from leptospira spp. And artificially contaminated with other microorganisms. The development of alternative methodologies to those currently imported, as well as nanostructured materials in Brazilian soil is strategic for the technological advance of the country. The methodological sequence described here can be used in bacteriology and immunology laboratories and enhances in vitro isolation of pathogens and their identification through molecular tests.